Polarized growth an essential plant process; vesicles are trafficked and fused to the tip of growing cells. The exocyst complex directs vesicles to the plasma membrane. This project aimed to create a Sec6-GFP fusion to image exocyst movement in polarized Physcomitrella patens cells. Myosin XI is a motor protein hypothesized to traffic vesicles for tip growth. A yeast two hybrid screen produced RabE14 as a potential myosin-vesicle binding partner. RabE12, myosin XI and vesicles had similar localization during polarized growth. Binding assays showed low interactions of purified RabE14 and myosin XI. RabE12 cell lysate coimmunoprecipitation assays resulted in no binding. These data do not support RabE as the vesicle-associated myosin XI binding partner.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Butt, Allison Marie

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-041917-132300

Advisor

• Heilman, Destin
• Vidali, Luis

Year

• 2017

Sponsor

• UMass Medical School

Date created

• 2017-04-19

Resource type

• Major Qualifying Project

Major

• Interdisciplinary

Rights statement

• In Copyright