Faculty Advisor

Heilman, Destin

Faculty Advisor

Vidali, Luis

Abstract

Polarized growth an essential plant process; vesicles are trafficked and fused to the tip of growing cells. The exocyst complex directs vesicles to the plasma membrane. This project aimed to create a Sec6-GFP fusion to image exocyst movement in polarized Physcomitrella patens cells. Myosin XI is a motor protein hypothesized to traffic vesicles for tip growth. A yeast two hybrid screen produced RabE14 as a potential myosin-vesicle binding partner. RabE12, myosin XI and vesicles had similar localization during polarized growth. Binding assays showed low interactions of purified RabE14 and myosin XI. RabE12 cell lysate coimmunoprecipitation assays resulted in no binding. These data do not support RabE as the vesicle-associated myosin XI binding partner.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2017

Major

Biology and Biotechnology

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Chemistry and Biochemistry

Advisor Department

Biology and Biotechnology

Available for download on Friday, April 19, 2019

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