Dr. Max Nibert
Current research suggests that gut-associated lymphoid tissue (GALT) may be one of the primary HIV reservoirs in the body. Recent advances in the field of Orthoreovirus reverse genetics allow for the possibility of this GI targeting virus to be used in the creation of an HIV vaccine. We provide support that based on the segmented nature of the Orthoreovirus genome, 5' duplication of the segments ORF is the best possibility for insertion of the HIV p24 capsid protein coding sequence for use as a vaccine antigen. We show there exists a maximum practical length for the length of code which can be added via the 5' duplication method, over which, recombination to original wild type occurs. This limit is an important consideration for future work and design with the Orthoreovirus.
Worcester Polytechnic Institute
Biology and Biotechnology
Major Qualifying Project
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Chemistry and Biochemistry