Faculty Advisor

Page, Raymond L.

Abstract

Current skeletal muscle cell therapy systems have limited effectiveness due to poor proliferative capability. The cells are not functional enough to be implanted into a critical size muscle defect as a therapy for volumetric muscle loss. Altering in vitro factors, such as mitogens and oxygen tension, can have a favorable effect on the cells ability to retain myogenic potential. Individual system parameters were characterized through experimental testing of culture conditions, media composition, and cell kinetics. Our team designed, engineered, and validated a final scalable cell expansion system for proliferating myogenic cells in vitro.

Publisher

Worcester Polytechnic Institute

Date Accepted

May 2014

Major

Biomedical Engineering

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biomedical Engineering

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