The aim of this project was to determine if the WIF-B cell line, a mouse hepatoma-human fibroblast hybrid, could be used as a model system for bile induced hepatocyte apoptosis as an alternative to primary cell culture. This is of particular interest because bile acid induced hepatocyte apoptosis accompanies cholestatic liver disease as a result of impaired bile flow. The results of this study revealed that WIF-B cells do not undergo apoptosis following exposure to bile acids. This is most likely due to the lack of significant membrane transport of bile acids in this cell line.
Worcester Polytechnic Institute
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Biology and Biotechnology