Faculty Advisor

Gibson, Daniel Gilbert

Faculty Advisor

Ryder, Elizabeth F.

Abstract

The dig-1 gene in Caenorhabditis elegans encodes an adhesion molecule in the immunoglobulin superfamily that is important to nervous system development. To determine time and location of DIG-1 protein expression, wild type and mutant gravid adults were embedded and sectioned. Sections were held to glass slides using heat and stained with two antibodies specific to DIG-1. Mutant strain n1480, which is missing the C-terminus portion of the protein which contained the CON-3 binding site, was used to compare the specificity of the anti-CON-3 against wild type. Initial results suggest that DIG-1 is expressed only in the developing embryos of C. elegans. The specificity of the anti-CON-3 was shown in that it showed little if no binding in the mutant n1480, which expresses a truncated DIG-1 protein and is hypothesized to not have the CON-3 binding site. Fixing, embedding, and molding techniques were also tested to determine efficiency of processes.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2003

Major

Biology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

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