Gibson, Daniel Gilbert
Ryder, Elizabeth F.
The dig-1 gene in Caenorhabditis elegans encodes an adhesion molecule in the immunoglobulin superfamily that is important to nervous system development. To determine time and location of DIG-1 protein expression, wild type and mutant gravid adults were embedded and sectioned. Sections were held to glass slides using heat and stained with two antibodies specific to DIG-1. Mutant strain n1480, which is missing the C-terminus portion of the protein which contained the CON-3 binding site, was used to compare the specificity of the anti-CON-3 against wild type. Initial results suggest that DIG-1 is expressed only in the developing embryos of C. elegans. The specificity of the anti-CON-3 was shown in that it showed little if no binding in the mutant n1480, which expresses a truncated DIG-1 protein and is hypothesized to not have the CON-3 binding site. Fixing, embedding, and molding techniques were also tested to determine efficiency of processes.
Worcester Polytechnic Institute
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Biology and Biotechnology