Faculty Advisor

Ryder, Elizabeth F.

Abstract

The dig-1 gene is proposed to be involved in axon guidance during C. elegans embryogenesis. Immunohistochemistry was used to investigate the expression pattern of several dig-1 alleles. Preliminary results showed staining during embryogenesis using an antibody generated against DIG-1 protein; however, controls suggest the antibody may not be specific. Various methods for immunohistochemistry were utilized to determine the most effective protocol and conditions for RT-PCR were optimized for future research of the dig-1 gene.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2006

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

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