Faculty Advisor

Rulfs, Jill

Faculty Advisor

Ryder, Elizabeth F.

Abstract

The DIG-1 protein in Caenorhabditis elegans is involved in neurological development and may be a proteoglycan. Experiments including immunoblotting, western analysis, acid proteolysis and ammonium precipitation were conducted to examine the protein. Immunoblotting showed that antibodies could be used to recognize the protein's c-terminus, which is missing in the n1480 mutant. Western analysis revealed a fragment that might be useful in determining the structure of DIG-1. If this is the expected fragment it should contain the glycosaminoglycans common in proteoglycans.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2004

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

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