Ryder, Elizabeth F.
The DIG-1 protein in Caenorhabditis elegans is involved in neurological development and may be a proteoglycan. Experiments including immunoblotting, western analysis, acid proteolysis and ammonium precipitation were conducted to examine the protein. Immunoblotting showed that antibodies could be used to recognize the protein's c-terminus, which is missing in the n1480 mutant. Western analysis revealed a fragment that might be useful in determining the structure of DIG-1. If this is the expected fragment it should contain the glycosaminoglycans common in proteoglycans.
Worcester Polytechnic Institute
Major Qualifying Project
Access to this report is limited to members of the WPI community. Please contact a project advisor or their department to request access
Restricted-WPI community only
Biology and Biotechnology