Faculty Advisor

Wobbe, Kristin K.


The effects of Turnip crinkle virus (TCV) on the disease signaling of Colombia-0 Arabidopsis thaliana was examined in this MQP. Following systemic infection of Col-0 plants by the virus, the plants were inoculated with both avirulent and non-avirulent Pseudomonas syringae pv. glycinea in order to study the hypersensitive response (HR) of the plants to the bacteria. The second messenger, hydrogen peroxide, was quantified in planta as an indicator of the plants' response. This research has suggested that TCV infection increases hydrogen peroxide accumulation in response to the introduction of all factors tested; mock inoculation solution, avirulent and non-avirulent bacteria. Furthermore, infected plants do not seem to distinguish between avirulent and non-avirulent pathogens in their response at the hydrogen peroxide stage in the HR pathway.


Worcester Polytechnic Institute

Date Accepted

January 2003



Project Type

Major Qualifying Project


Restricted-WPI community only

Advisor Department

Chemistry and Biochemistry