Faculty Advisor

Argüello, José M.

Abstract

The goal of this MQP was to develop the necessary methodology to initiate the characterization of six heavy metal transport ATPases found in Arabidopsis thaliana. Growth of Arabidopsis thaliana in an MS salt media, optimal subtoxic metal ion level (50 uM CuSO4, 50 uM ZnSO4, and 10' uM CdCl2), And RNA extraction were standardized. Two probes were designed for two genes (AAD29115), AAD12041). A Northern blot of the target genes was performed.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2001

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Chemistry and Biochemistry

Advisor Program

Chemistry and Biochemistry

Share

COinS