Currently, there are no reliable devices for high-throughput drug screening or testing for antiangiogenic drugs. 3D cultures hold promise in effectively mimicking the in vivo conditions without the high cost of animal models. This project was focused on developing a device capable of creating reliable 3D microenvironment for use with multiple cell types. We have developed a 3D culture system that can be adapted for high throughput analysis in multi-well formats. Our device allows for the creation of multiple hydrogel layers containing cells where angiogenesis initiated as a result of chemotaxis due to secretion of angiogenic factors by tumor cells can be analyzed. The device is intended to be used as a drug testing platform for antiangiogenic drugs and to study other biological phenomena.
Worcester Polytechnic Institute
Major Qualifying Project
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