Faculty Advisor

Rolle, Marsha W.

Abstract

Cyclic mechanical loading improves strength and extracellular matrix (ECM) synthesis in tissue engineered blood vessels (TEBV). The goal of this project was to design a device to impart cyclic circumferential stretch on cell-derived TEBV rings cultured on flexible silicone tubing. Our device cyclically displaces water (75 uL volume) to inflate tubing at a frequency of 1 Hz. During static inflation tests, the tubing diameter increased by 10+/-1.6%. TEBV rings were loaded onto silicone tubing and subjected to mechanical conditioning for 3-7 days. Our device conditioned samples in an incubator at 10% distension at a frequency of 1 Hz. Static and conditioned tissues remained viable and uncontaminated and exhibited high cell densities and increased thicknesses from 3 to 7 days.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2011

Major

Biomedical Engineering

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biomedical Engineering

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