Faculty Advisor

Adams, David S.


The Human Papillomavirus E2 protein is involved in multiple processes including transcriptional regulation of viral early genes and the stable maintenance of viral genomes. E2 has been shown to associate with mitotic chromosomes. However, the mechanism of this interaction. is unknown. Previously, E2 was shown to bind to protein CHLr1, a DNA helicase required for high fidelity chromosome maintenance during mitotic segregation. In this project, E2 mutants that do not bind to CHLr1 were isolated using the yeast two-hybrid system. Analysis of such mutants will help in determining the functional significance of the interaction.


Worcester Polytechnic Institute

Date Accepted

January 2004


Biology and Biotechnology

Project Type

Major Qualifying Project


Restricted-WPI community only

Advisor Department

Biology and Biotechnology