Faculty Advisor

Adams, David S.

Abstract

The murine antibody, 125-2H, is a potent neutralizer of interleukin (IL-18), which is associated with inflammation. Because of its murine origins it is not a clinical candidate, but its IL-18 neutralizing potency is the standard to which human monoclonal antibodies to IL-18 are held. To determine the amino acid residues most essential to its interaction with IL-18, alanine scan mutagenesis was performed on the third complementarity-determining regions (CDR3) of the light and heavy chains. Following testing in a KG-1 assay for IL-18 neutralizing ability, it was determined that the alanine substitutions at four CDR3 amino acid residues of the heavy chain are most essential for IL-18 binding.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2002

Major

Biotechnology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

Advisor Program

Biology and Biotechnology

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