Faculty Advisor

Bagshaw, Joseph C.

Abstract

The objective of this Major Qualifying Project was to design and synthesize an artificial gene encoding a lobster vitellogenesis-inhibiting hormone (VIH) and then express that gene in host yeast cells. Based on the amino acid sequence of VIH, a set of oligonucleotides were designed and optimized for the codon bias of the yeast expression system, P. pastoris. The oligonucleotides were assembled into a single DNA molecule, cloned into a plasmid vector and sequenced, however, expression of the hormone is ongoing.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2003

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

Advisor Program

Biology and Biotechnology

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