Faculty Advisor

Adams, David S.

Abstract

Dengue virus studies have shown that viral-encoded surface protein NS1G is linked to the host cell membrane via a GPI linkage and may exist in lipid rafts. Lipid raft formation may be required for the viral induction of host IL-8. This project investigated this hypothesis by expressing NS1 in both HEK293A and HeLa cells followed by treatment with methyl beta cyclodextrin to block lipid raft formation. The levels of secreted IL-8 were assayed using a luciferase reporter under the control of an IL-8 promoter. The results confirm that lipid raft formation is required for IL-8 promoter activation.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2010

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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