Faculty Advisor

Wobbe, Kristin K.

Abstract

The presence of Turnip crinkle virus (TCV) in Arabidopsis thaliana plants has previously been shown to suppress the ability of these plants to produce a hypersensitive response (HR) upon inoculation with pathogens that would normally elicit this defense response. The ecotype Colombia-O was examined using wild type TCV and non-pathogenic strains of Pseudomonas syringae pv. glycinae containing virulence factor avrRpt2. Transgenic lines of A. thaliana that express the TCV proteins p8 or p9 were also examined in an attempt to determine if these proteins play a role in suppression of the HR. In addition, assays were done to determine if the inhibition of the HR correlated with inhibition of pathogen growth in the plant. Finally, PR-1 protein expression was inspected to determine if TCV also played a role in inhibition of the plants' ability to initiate systemic acquired resistance (SAR).

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2002

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Chemistry and Biochemistry

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