Faculty Advisor

Billiar, Kristen

Faculty Advisor

Burnham, Nancy A.

Abstract

Despite its common use in cell culture studies, polyacrylamide gel's mechanical properties remain unclear. To characterize this gel fully, atomic force microscopy, uniaxial testing, rheology, and ball indentation were used. The range of the elastic moduli was 1.9-7.5 kPa and 18.1-39.4 kPa, for 5%/0.025% and 8%/0.08% gel concentrations, respectively, and a Poisson's ratio of 0.45 was found. Future research utilizing polyacrylamide as a cell culture substrate should indicate how the gel was characterized and acknowledge the uncertainty in stiffness values.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2006

Major

Biomedical Engineering

Major

Physics

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biomedical Engineering

Advisor Department

Physics

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