Adams, David S.
Expressive Constructs, Inc
The purpose of this MQP was to clone and express the gene for the Salmonella secreted virulence protease, SipB, as a first step in developing a sensitive fluorescent peptide substrate assay for rapidly detecting Salmonella contamination. A test for such a protease would be specific for virulent Salmonella, not dead or latent bacteria. The SipB gene was amplified by PCR from Salmonella genomic DNA, cloned into an expression plasmid, and verified by sequence analysis. Immunoblots verified the presence of the expressed protein E. coli lysates. Induced cells showed more SipB in the insoluble protein fraction versus the soluble.
Worcester Polytechnic Institute
Major Qualifying Project
Access to this report is limited to members of the WPI community. Please contact a project advisor or their department to request access
Restricted-WPI community only
Biology and Biotechnology