Faculty Advisor

Adams, David S.

Abstract

The purpose of this MQP was to clone and express the gene for the Salmonella secreted virulence protease, SipB, as a first step in developing a sensitive fluorescent peptide substrate assay for rapidly detecting Salmonella contamination. A test for such a protease would be specific for virulent Salmonella, not dead or latent bacteria. The SipB gene was amplified by PCR from Salmonella genomic DNA, cloned into an expression plasmid, and verified by sequence analysis. Immunoblots verified the presence of the expressed protein E. coli lysates. Induced cells showed more SipB in the insoluble protein fraction versus the soluble.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2000

Major

Biotechnology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

Advisor Program

Biology and Biotechnology

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