Adams, David S.
The purpose of this MQP was to obtain sequence information from the gag and env genes of a Long Term Non-Progressor (LTNP) HIV-1 patient to identify candidate mutations potentially responsible for the LTNP phenotype. PCR conditions were optimized to amplify portions of the gag and env genes previously determined to represent prime mutagenic sites. The gag PCR reactions produced discrete bands of the expected size, however all env reactions produced smears. PCR products were cloned into a T-tailed plasmid. Subsequent screening produced bands of exactly the expected sizes, including those cloned from the smears. Unfortunately, plasmid sequencing produced only low-quality information, so no sequence comparisons were obtained.
Worcester Polytechnic Institute
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Biology and Biotechnology