Faculty Advisor

Adams, David S.

Abstract

The purpose of this project was to clone and express several multimerized antimicrobial peptides in a bacterial system using recombinant DNA technology. Plasmids encoding multimerized peptides indolicidin and PGQ were initially cloned into pUC vector, then subsequently subcloned into the expression vector pQE-30. DNA sequencing of the genes in pQE-30 indicated that no point mutations were present and all inserts were in the correct reading frame. Results indicated that five successful clones were engineered. However peptide expression was not observed by SDS-PAGE (for total lysates or nickel purified proteins) or by colony blots. These short peptides should eventually provide an adequate system for killing both gram positive and negative bacteria as well as fungi for use in food preparation surfaces, antimicrobial textiles for biological agent decontamination and extended wear textiles.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2001

Major

Biotechnology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

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