Title

HIV-Tropism

Faculty Advisor

Adams, David S.

Abstract

Developing a vaccine against HIV has been very difficult mainly because HIV is highly variable. Many different avenues of overcoming this difficulty are being researched, including interfering with HIV attachment to host cells. In all stages of HIV infection, the viral envelope glycoprotein (gp120) is necessary for attachment to CD4 surface proteins on human cells. To identify potential reagents for blocking gp120/CD4 interaction, a random peptide library was constructed in search of a peptide capable of binding to gp120's CD4 binding site. The library was screened using an E. coli two-hybrid system, and yielded 53 peptides that interact with a mimic of gp120's CD4 binding site.

Publisher

Worcester Polytechnic Institute

Date Accepted

March 2010

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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