Faculty Advisor

Adams, David S.


The purpose of this project was to evaluate the immune response produced in rabbits by immunization with DNA vaccines. The vaccines are intended to raise specific antibodies, which in theory can provide protection against infection with HIV. Genes encoding various HIV envelope glycoproteins gp 120 had previously been cloned into a plasmid vector creating a DNA vaccine, which was then used to vaccinate rabbits using a gene gun. Various vaccines were tested, each containing gp 120 from a different strain of HIV, or multiple gp 120 genes from different strains in the same vector. In this project the sera from the rabbits were examined and the antibody titer raised against the foreign protein was evaluated by ELISA assay. The data showed that an antibody response to gp 120 was successfully produced in rabbits by immunization with each vaccine.


Worcester Polytechnic Institute

Date Accepted

January 2001



Project Type

Major Qualifying Project


Restricted-WPI community only

Advisor Department

Biology and Biotechnology