Faculty Advisor

Adams, David S.

Abstract

The purpose of this project was to produce antibodies against specific neurotransmitter receptors for the long-range purpose of analyzing their effects on various chemical brain interactions. A bioinformatics approach was used to design immunogenic peptide specific for the transmembrane domains of seven receptors. Mouse and Rat brain cDNA libraries were used as PCR templates to amplify receptor gene fragments for ligation into expression plasmids. Restriction mapping indicated no positives, thus an alternative approach was utilized in which the peptides were synthesized synthetically and injected into rabbits. This latter approach produced a high titer antibody response as assayed by ELISAs.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2002

Major

Biotechnology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

Advisor Program

Biology and Biotechnology

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