Effects of methylation on PES1 expression and exon identification.

Faculty Advisor

Adams, David S.


The purpose of this project was to use RT-PCR to determine whether methylation of the PES1 tumor suppressor promoter is responsible for its decreased expression in prostate tumor cells. PES1 expression was found to increase in tumor cells pre-treated with a methylase blocking agent, indicating that methylation of the CpG islands in its promoter may indeed be responsible for its under expression. In addition, the results from this project have identified two exon regions located on chromosome X in a putative gene responsible for prostate cancer. This research was conducted in the Department of Surgery, Urology Division at University of Massachusetts Medical Center (Worcester, Massachusetts) under the direction of Dr. Shuk-Mei Ho.


Worcester Polytechnic Institute

Date Accepted

January 2000



Project Type

Major Qualifying Project


Restricted-WPI community only

Advisor Department

Biology and Biotechnology

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