Bagshaw, Joseph C.
The scope of this report is to document the methodology used in creating nonsense codons in plasmid DNA by site directed mutagenesis as well as the application in site-specific incorporation of non-natural amino acids into target peptides. A technique to use artificial suppressor tRNA in an in vitro expression system based on a Micrococcus luteus S30 extract is suggested as an alternative to stop codon suppression. Site-specific protein mutagenesis has wide reaching implementations in biochemistry.
Worcester Polytechnic Institute
Major Qualifying Project
Access to this report is limited to members of the WPI community. Please contact a project advisor or their department to request access
Restricted-WPI community only
Biology and Biotechnology