Faculty Advisor

Heilman, Destin

Center

University of Massachusetts Medical Center

Abstract

Differences in innate immune response to L. Monocytogenes infection are associated with genetic polymorphisms, and are studied using inbred mouse strains. Differences in susceptibility in BALB/c and C57BL/6J strains have been associated with the L2 locus of chromosome 13. Cathepsin L, which is located within this locus, was identified as a candidate susceptibility gene based on its differential expression in macrophages from these mice. A construct was created to analyze cathepsin L expression in macrophages. A novel mechanism of Ctsl regulation was discovered, which appeared to be controlled at a transcriptional level based on introduction of dsDNA or LPS. There were differences in this regulation pathway between the two mouse strains.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2011

Major

Chemistry

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

Project Center

University of Massachusetts Medical Center

Your accessibility may vary due to other restrictions.

Share

COinS