UMMC / University of Massachusetts Medical Center
Professor Victor Boyartchuk
Differences in innate immune response to L. Monocytogenes infection are associated with genetic polymorphisms, and are studied using inbred mouse strains. Differences in susceptibility in BALB/c and C57BL/6J strains have been associated with the L2 locus of chromosome 13. Cathepsin L, which is located within this locus, was identified as a candidate susceptibility gene based on its differential expression in macrophages from these mice. A construct was created to analyze cathepsin L expression in macrophages. A novel mechanism of Ctsl regulation was discovered, which appeared to be controlled at a transcriptional level based on introduction of dsDNA or LPS. There were differences in this regulation pathway between the two mouse strains.
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry