Diffusion studies allow scientists to gain a better understanding of the function and structure of membranes. However, these diffusion studies can be time consuming and costly when using current devices on the market. Our goal was to design and validate an affordable high-throughput molecular diffusion system to characterize membranes. Our device was validated using glucose, bovine serum albumin (BSA), and gamma globulin. The calculated coefficients were 5.58x10-6 cm2/s, 7.08x10-8 cm2/s, and 4.83x10-8 cm2/s for glucose, BSA, and gamma globulin, respectively. Diffusion coefficients were shown to be the same for our device and a marketed device and are comparable to published values, suggesting that our device will serve as a facile tool to rapidly characterize engineered membranes.
Worcester Polytechnic Institute
Major Qualifying Project