Faculty Advisor

Crusberg, Theodore C.

Abstract

The vast amount of bacteria and difficulty in their culturability has impeded progress in classifying and identifying these many unknown species. The purpose of this project was to develop a micro-array blot system to determine the presence and eventually the relative amounts of bacteria in virtually any environment. A hybridization protocol was developed here using florescent (6-FAM) tagged, oligonucleotide probes with fully extracted samples of ribosomal RNA from Proteobacteria of the alpha, beta and gamma sub-classes.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2002

Major

Biology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

Advisor Program

Biology and Biotechnology

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