Crusberg, Theodore C.
The vast amount of bacteria and difficulty in their culturability has impeded progress in classifying and identifying these many unknown species. The purpose of this project was to develop a micro-array blot system to determine the presence and eventually the relative amounts of bacteria in virtually any environment. A hybridization protocol was developed here using florescent (6-FAM) tagged, oligonucleotide probes with fully extracted samples of ribosomal RNA from Proteobacteria of the alpha, beta and gamma sub-classes.
Worcester Polytechnic Institute
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Biology and Biotechnology