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Mapping DNA Replication in the Yeast Species SACCHAROMYCES CEREVISIAE

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DNA replication is an essential function that allows all living organisms to maintain life. The timing of replication origin firings drives replication timing. One way timing is regulated is by loading of the replicative helicase complex, MCM. An origin is more likely to fire earlier when more MCM, a hexamer that aids in the formation of the replication fork, is loaded by the Origin Recognition Complex. The goal of this MQP is to examine the number of MCM complexes loaded on single replication origins before S phase is initiated. In order to identify the specific number of MCM complexes that are loaded on any given origin, a S. cerevisiae strain was built. This plasmid will be captured in a flow cell in order to count the number of MCMs loaded on single plasmids containing known origins.

  • This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
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  • E-project-042717-142337
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  • 2017
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  • 2017-04-27
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