Faculty Advisor

Rao, Reeta Prusty

Abstract

DNA replication is an essential function that allows all living organisms to maintain life. The timing of replication origin firings drives replication timing. One way timing is regulated is by loading of the replicative helicase complex, MCM. An origin is more likely to fire earlier when more MCM, a hexamer that aids in the formation of the replication fork, is loaded by the Origin Recognition Complex. The goal of this MQP is to examine the number of MCM complexes loaded on single replication origins before S phase is initiated. In order to identify the specific number of MCM complexes that are loaded on any given origin, a S. cerevisiae strain was built. This plasmid will be captured in a flow cell in order to count the number of MCMs loaded on single plasmids containing known origins.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2017

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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