Pins, George D
A major challenge in tissue engineering is overcoming the diffusion limit of oxygen and nutrients in engineered tissue. The team designed and developed perfusable microengineered vasculature within a fibrin hydrogel. This vasculature was created using a gelatin sacrificial mold that was tunable and continuously perfused for up to 24 hours. Additionally, a bioreactor system with a custom microfluidic chamber was developed to perfuse and study tissue constructs. The combined system maintained viability of C2C12 cells seeded greater than 1200 μm from the edge of the perfused vasculature. This project suggests that microengineered vasculature formed using gelatin sacrificial molds and cultured in a microfluidic chamber have potential to be used in cardiac tissue engineering.
Worcester Polytechnic Institute
Major Qualifying Project
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