Faculty Advisor

Buckholt, Michael Allan

Faculty Advisor

Hunter, Allison

Abstract

Due to the growing US Hispanic population, there is an agricultural incentive to grow the El Salvadoran leguminous herb "Chipilin" (Crotalaria longirostrata) on New England farms. Legumes require a symbiotic relationship with rhizobia in order to utilize atmospheric nitrogen. However, it is not known which strains of rhizobia are appropriate for establishing nitrogen-fixing nodules in Chipilin. Six strains of rhizobia were evaluated for increased plant yield and decreased nitrogen application. Upon harvest, Western Blotting was used to successfully probe for leghemoglobin, a protein indicator of nitrogen fixation.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2010

Major

Biology and Biotechnology

Major

Professional Writing

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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