Faculty Advisor

Adams, David S.

Center

University of Massachusetts Medical Center

Abstract

E3 ubiquitin ligases, such as SCFGrr1, are enzymes that add ubiquitin chains to proteins targeting them to the proteasome for degradation. Deubiquitinating enzymes (DUBs) can counteract this activity by removing ubiquitin chains and thus rescue proteins from degradation. Our goal was to develop genetic and biochemical screening approaches to identify DUB substrates, and thus learn more about DUBs that may contribute to human disease. Our data suggests that the yeast DUBs Ubp3 and Ubp12 affect the stability of the Grr1 targets Cln2, Cln3, and Gic2.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2012

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

Project Center

University of Massachusetts Medical Center

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