Faculty Advisor

Wobbe, Kristin K.

Abstract

To gain a better understanding of the regulation of artemisinin biosynthesis, this project was designed to clone several fragments encoding putative sesquiterpene cyclases. Utilizing two gene amplification strategies, one cDNA fragment was produced by RT-PCR and three genomic fragments from PCR were cloned and sequenced. The analyses of these fragments showed regions of high homology to sesquiterpene cyclase genes and other regions of very low homology. The regions of low homology in some cases are likely to be intron sequences which would not be present in cDNA sequences.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2001

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Chemistry and Biochemistry

Advisor Program

Chemistry and Biochemistry

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