Wobbe, Kristin K.
To gain a better understanding of the regulation of artemisinin biosynthesis, this project was designed to clone several fragments encoding putative sesquiterpene cyclases. Utilizing two gene amplification strategies, one cDNA fragment was produced by RT-PCR and three genomic fragments from PCR were cloned and sequenced. The analyses of these fragments showed regions of high homology to sesquiterpene cyclase genes and other regions of very low homology. The regions of low homology in some cases are likely to be intron sequences which would not be present in cDNA sequences.
Worcester Polytechnic Institute
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Chemistry and Biochemistry