Adams, David S.
Project Pediatric Immunology Lab, UMass Medical School
Two HIV-1 clones that have different cytopathogenicity were previously isolated from a single patient. In this MQP PCR amplification of the env, gag, nef, and vpr regions of the two clones was performed. Sequencing of the two clones was done to identify sequence variations potentially responsible for the difference in cytopathogenicity. The vpr sequence of the noncytopathic clone revealed an early stop codon resulting in a truncated protein. This is a significant difference and is a likely candidate for affecting the cytopathogenicity.
Worcester Polytechnic Institute
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Biology and Biotechnology