Faculty Advisor

Heilman, Destin

Abstract

Early embryogenesis and cell fate specification of Caenorhabditis elegans is mainly driven by post-transcriptional regulation, involving RNA binding-proteins that bind to the 3Â’ UTRs of maternal mRNAs. Regulation of this binding activity is crucial to the maturation of the zygote since this process ensures proper formation of the body plan. The goal of this project is to understand the regulation of different 3Â’ UTRs by different RNA binding proteins. To study this regulation, it is necessary to study the expression patterns driven by 3Â’UTRs using transgenic strains. To achieve this, we used a library MosSCI approach to generate multiple reporter strains in a faster way.

Publisher

Worcester Polytechnic Institute

Date Accepted

May 2014

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

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