Understanding the genetic basis of plant growth is important for agricultural improvements. Here, the intracellular localization of CLoG1, a novel protein identified in a temperature sensitive loss-of-growth genetic screen in Physcomitrella patens is investigated. An RNAi complementation assay was used to demonstrate that mEGFP tagged CLoG1 remains functional. Following this, stable lines expressing CLoG1:mEGFP fusions were generated. Imaging by confocal microscopy revealed that CLoG1 associates with the microtubule cytoskeleton, including the mitotic spindle and phragmoplast. A nuclear counting assay was conducted to investigate the participation of CLoG1 in cell division. Though there was no evidence of multi-nucleated cells, the mutation causes a decrease in the number of cells.
Worcester Polytechnic Institute
Biology and Biotechnology
Major Qualifying Project
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