Faculty Advisor

Adams, David S.

Center

UMMC / University of Massachusetts Medical Center

Abstract

Early embryogenesis cell fate specification in Caenorhabditis elegans is driven primarily by post-transcriptional regulation, in which RNA-binding proteins bind to the 3' UTRs of complementary mRNAs. POS-1 is a RNA-binding protein necessary for germline specification in early embryogenesis. Transgenic worm strains with possible POS-1 binding sites were used to study the underlying mechanisms of stem cell fate regulation by POS-1. We also modified the Mos1-mediated single-copy insertion method to increase production efficiency of transgenic strains of C. elegans.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2011

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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