Faculty Advisor

Wobbe, Kristin K.


Viral proteins are essential for cell-to-cell spread of plant viruses, although this process is not yet fully understood. Turnip crinkle virus (TCV) is a 30nm icosahedral plant virus of the genus Carmovirus containing five open reading frames (ORFs) (Carrington et al., 1989, Hacker et al.,1992). The two proteins encoded by TCV (p8 and p9) are required for the cell-to-cell movement process (Li et al., 1998). Both of these proteins, p8 and p9, must be in the same cell in order for TCV to achieve movement. (Li et al., 1998). Additionally, given current models for plant virus movement, it seems likely that these proteins interact with each other. A number of assays used to detect protein-protein interactions were tested to identify potential interactions between p8 and p9.


Worcester Polytechnic Institute

Date Accepted

January 2000



Project Type

Major Qualifying Project


Restricted-WPI community only

Advisor Department

Chemistry and Biochemistry