Faculty Advisor

Ryder, Elizabeth F

Abstract

Early in C. elegans development, several neurons must migrate to specific locations within the body. Mutations in mig-10 result in incomplete migration of several neurons, as well as defects in egg laying and excretory cell development. We tested the ability of each of the two mig-10 splice forms to rescue the egg laying defect; partial rescue was observed. These results indicate that perhaps more than one isoform of the MIG-10 protein must be expressed simultaneously in multiple cells in order to achieve total mutant rescue. To test the hypothesis that expression in specific neurons is required for rescue of the migration and excretory cell defects, constructs were made to express each mig-10 splice form in a particular neuron.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2006

Major

Biology and Biotechnology

Major

Humanities and Arts

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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