Viral proteins produced by the Chicken Anemia Virus and the Torque Teno Virus have the ability to selectively induce p53 independent apoptosis in cancer cells. Each protein specifically localizes in cancer cells; CAV VP3 to the nucleus and TTV VP3 to the cytoplasm. The importance of protein localization in respect to killing capacity is still unknown. Cell viability and localization assays were utilized to evaluate the killing capacity of these proteins in opposite cellular compartments. The mutant construct of CAV VP3 has altered amino acids 86-88 and 116-118 to localize in the cytoplasm. This mutant locates to the cytoplasm and does not induce apoptosis. TTV VP3 will be pushed to the nucleus by fusing a GFP-TTV VP3 with a SV40NLS. Efforts to obtain this construct are ongoing.
Worcester Polytechnic Institute
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Chemistry and Biochemistry
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