Faculty Advisor

Adams, David S.

Abstract

Virulent E. coli strain 0157:H7 produces Shiga toxins as part of its mechanism of infection. Shiga toxin-2 is more virulent than toxin-1. The purpose of this MQP was to investigate this difference by testing the virulence of recombinant toxins whose subunits have been exchanged. To accomplish this, a production system for toxin subunits A and B had to be devised. This was originally attempted using suicide vector mutagenesis with no success, but was partially achieved using PCR-mediated gene replacement. The produced toxins will eventually be tested in a gnotobiotic piglet model for E. coli infection.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2001

Major

Biotechnology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

Advisor Program

Biology and Biotechnology

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