Faculty Advisor

Gibson, Daniel Gilbert

Abstract

We assayed three amino acid neuromuscular transmitters (1 Molar in artificial seawater) on muscles of the chelicerate arthropod, Limulus polyphemus. Muscle fiber responses in semi-intact first-instar juvenile walking legs were recorded via videomicroscope. Glutamate, the primary excitor of insect and crustacean muscle, and Aspartate, sometimes co-released with Glutamate (lobster), each elicited strong rhythmic muscle contractions, but a 1:1 mixture of the two elicited even stronger contractions. Although inhibitory on insect and crustacean muscles, y-amino butyrate (GABA) was excitatory in Limulus.

Publisher

Worcester Polytechnic Institute

Date Accepted

January 2001

Major

Biotechnology

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biology and Biotechnology

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