Faculty Advisor

Gaudette, Glenn R

Abstract

HCN gene modified Human Mesenchymal Stem Cells (hMSCs) have shown the ability to restore pacemaker function in canine and murine models. However, stem cell migration from the delivery site is a major concern. To address this problem, the team designed a device to prevent migration but still allow communication between the stem cells and native cardiomyocytes. Scaffold pore size, fiber diameter and biomaterial selection were necessary to determine. The team conducted a pore size migration assay, cell deflection calculations, gap junction formation assay and cell viability assays. The results from the tests led to the design of a two part polyurethane scaffold that prevents migration of stem cells, allows gap junction formation through pores and is packaged for minimally invasive delivery.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2009

Major

Biomedical Engineering

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biomedical Engineering

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