Gaudette, Glenn R
HCN gene modified Human Mesenchymal Stem Cells (hMSCs) have shown the ability to restore pacemaker function in canine and murine models. However, stem cell migration from the delivery site is a major concern. To address this problem, the team designed a device to prevent migration but still allow communication between the stem cells and native cardiomyocytes. Scaffold pore size, fiber diameter and biomaterial selection were necessary to determine. The team conducted a pore size migration assay, cell deflection calculations, gap junction formation assay and cell viability assays. The results from the tests led to the design of a two part polyurethane scaffold that prevents migration of stem cells, allows gap junction formation through pores and is packaged for minimally invasive delivery.
Worcester Polytechnic Institute
Major Qualifying Project
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