This project explores the function of Core Binding Factor (CBF) in B-cell development, and its regulation on the recombination activating genes (RAG) that encode the B-cell antibody gene recombinase via Erag enhancer. A GFP reporter construct containing Erag and the RAG2 promoter was transfected into pro-B cells, and was mutated to block CBF binding. Leukemia cells and transfected pro-B cells were treated with CBF inhibitor to measure the effects of disrupted CBF function. The transcript levels of B-cell specific proteins of inhibitor-treated cells were measured by qPCR. The data indicate cell viability and key B cell protein expressions were decreased, but reporter activity was not decreased after inhibitor treatment. The mutant was obtained but was not yet transfected.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Huang, Zhao

Contributors

• Gerstein, Rachel

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042911-104646

Advisor

• Adams, David S.

Year

• 2011

Sponsor

• UMASS Medical Center

Date created

• 2011-04-29

Resource type

• Major Qualifying Project

Major

• Biology & Biotechnology

Rights statement

• In Copyright

Last modified

• 2023-10-09