Faculty Advisor

Adams, David S.


This project explores the function of Core Binding Factor (CBF) in B-cell development, and its regulation on the recombination activating genes (RAG) that encode the B-cell antibody gene recombinase via Erag enhancer. A GFP reporter construct containing Erag and the RAG2 promoter was transfected into pro-B cells, and was mutated to block CBF binding. Leukemia cells and transfected pro-B cells were treated with CBF inhibitor to measure the effects of disrupted CBF function. The transcript levels of B-cell specific proteins of inhibitor-treated cells were measured by qPCR. The data indicate cell viability and key B cell protein expressions were decreased, but reporter activity was not decreased after inhibitor treatment. The mutant was obtained but was not yet transfected.


Worcester Polytechnic Institute

Date Accepted

April 2011


Biology and Biotechnology

Project Type

Major Qualifying Project



Advisor Department

Biology and Biotechnology