Faculty Advisor

Buckholt, Michael Allan

Abstract

Two bacterial samples, referred to as 14-29 & 15-6, were chosen from among several putative antibiotic-producing isolates originally discovered in a WPI laboratory course titled “Microbes to Molecules: Crowdsourcing Novel Antibiotic Discovery” from 2014 to present. Potential antibiotics were extracted from the samples and assayed for efficacy against E. coli and B. subtilis and characterized by HPLC. Antibiotic activity was visible to varying degrees on assay plates for each sample. Colony inhibition was demonstrated by each sample, but only 15-6 demonstrated inhibition from extraction. Identification of these antibiotics will determine their novelty and potential application for treatment of infection.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2018

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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