Faculty Advisor

Albrecht, Dirk

Faculty Advisor

Radhakrishnan, Pradeep

Abstract

Transgenic C. elegans are created via microinjection of plasmid DNA, a process that has a short time frame of 10min in its current state. An new method developed in Prof. Albrecht's lab utilizes PEG to encapsulate the worms, increasing longevity to 24h or more, with a observed issue of the healthy shell becoming more durable, leading to difficulty puncturing. Our team developed an attachment to solve this problem. Our design was a 3D-printed attachment, utilizing vibration to provide 5-15 µm of movement in the needle. This motion was quantified by microscopy as 4 µm transversely, and 12 µm axially, which reduced the depression of the C. elegans cuticle from 67 µm to 27 µm. Our design was confirmed via successfully injecting dye into the C. elegans.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2018

Major

Biomedical Engineering

Major

Mechanical Engineering

Project Type

Major Qualifying Project

Accessibility

Restricted-WPI community only

Advisor Department

Biomedical Engineering

Advisor Department

Mechanical Engineering

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