Faculty Advisor

Ryder, Elizabeth F.

Abstract

Genetic interaction studies have identified mechanisms controlling tissue specific gene expression, but with limited scalability. A high throughput image analysis platform was designed in conjunction with a genetic construct. The construct marks the intestinal cells in Caenorhabditis elegans, using mCherry, and indicates promoter of interest expression using GFP. The computational system creates a mask using the mCherry signal and quantifies GFP fluorescence, outputting quantitative changes in intensity within the intestine.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2019

Major

Biology and Biotechnology

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Biology and Biotechnology

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