In this study, the binding of IQGAP1 to Akt1 and EGFR in the HeLa cell line was investigated using Fluorescence Lifetime Imaging Microscopy. It was confirmed that Akt1 and EGFR do interact in the presence of IQGAP1. Additionally, an increased interaction between PIP3 and EGFR was observed upon stimulation with EGF. This interaction was found to be mediated through IQGAP1 scaffolding, which suggests that the scaffold plays a key role in modulating the PI3K/Akt/mTOR signaling pathway's response to pro-migratory stimuli such as EGF.
Worcester Polytechnic Institute
Major Qualifying Project
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Chemistry and Biochemistry