Faculty Advisor

Arguello, Jose' M.

Faculty Advisor

Shell, Scarlet

Abstract

Mycobacterium tuberculosis is a bacterium that causes tuberculosis. Regulation of gene expression and mRNA degradation is important for the ability of the bacteria to survive the stressors of infection. A multiprotein complex called the degradosome is important for mRNA degradation in better-studied bacteria such as E. coli, where Ribonuclease E (RNase E) cleaves mRNA into fragments while using a scaffold domain to interact with other proteins involved in mRNA degradation. We used M. smegmatis, a non-pathogen relative of M. tuberculosis, as a model to define the putative scaffold domains of mycobacterial RNase E. Our results suggest that RNase E has two scaffold domains flanking a putative catalytic domain 493 amino acids in length.

Publisher

Worcester Polytechnic Institute

Date Accepted

April 2019

Major

Biochemistry

Project Type

Major Qualifying Project

Accessibility

Unrestricted

Advisor Department

Chemistry and Biochemistry

Advisor Department

Biology and Biotechnology

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